Protective effects of monoammonium glycyrrhizinate on fatty deposit degeneration induced in primary calf hepatocytes by sodium oleate administration in vitro. (5th December 2022)

• Record Type: Journal Article
• Authors: Zhang, Cai; Meng, Sudan; Shao, Qi; Wang, Xueying; Li, Chenxu; Chen, Wenbin; Li, Yuanxiao; Huang, Shucheng; Ma, Yanbo
• Is Part Of: Research in veterinary science. Volume 150(2022)
• Page Start: 213
• Page End: 223
• Publication Date: 2022-12-05
• Subjects: Monoammonium glycyrrhizinate -- Sodium oleate -- Hepatocytes -- Lipid metabolism -- Apoptosis; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2022.05.011
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: In this study we investigated the effect of MAG on fatty deposit-induced degeneration of primary calf hepatocytes induced by sodium oleate. Primary hepatocytes were isolated from dairy calves and cultured before allocation to the following treatment groups: control (untreated), model (starved for 12 h before treatment with 0.25 mM sodium oleate to induce steatosis-like changes, and the MAG group pretreated with MAG (0.1, 0.25, 0.5, and 1.5 mM) for 12 h before sodium oleate treatment (0.25 mM) for 12 h). To evaluate the effect of MAG on fat-induced degradation of primary hepatocytes, we evaluated lipid deposition, cell viability, apoptosis rate, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity in the culture supernatant, and expression of oxidant and antioxidant enzymes (LDH, MDA, GSH, and CAT), as well as the expression of lipid metabolism-related genes (PPARα, SREBP-1c, ChREBP, CPT1, CPT2, and MTP) and apoptosis-related genes (Cyt-c, Caspase 9, Caspase 8, Caspase 3, Bax, and Bcl-2). MAG significantly reduced lipid accumulation in hepatocytes induced by sodium oleate ( P < 0.05), increased cell viability, decreased the apoptosis rate (P < 0.05), and significantly decreased ALT and AST activity in the culture supernatant ( P < 0.05). MAG significantly decreased MDA levels in cells and LDH levels in the culture supernatant, while GSH and CAT levels were increased ( P < 0.05). MAG significantly increased the expression of the lipid transport- and metabolism-related genes MTP, PPARα, CPT 1 and CPT 2, and decreased ChREBP expression ( P < 0.05). At concentrations higher than 0.25 mM, MAG significantly decreased SREBP-1c expression ( P < 0.05). MAG significantly decreased the expression of the apoptosis-related genes Cyt-c, Caspase 9, Caspase 8, Caspase 3 and Bax, while Bcl- 2 expression was increased ( P < 0.05). These findings demonstrate that MAG improves the antioxidant capacity of hepatocytes and effectively reduces lipid deposition by inhibiting the expression of lipid metabolism- and apoptosis-related genes. Highlights: In this study, we established a model of sodium oleate-induced steatosis in primary dairy cow hepatocytes in vitro. We pretreated the model cells with different concentrations of MAG to observe its effects and explore the mechanism. This will lay a foundation for further explorations of MAG for the prevention of fatty liver disease in dairy cows.