Roles of ERβ and GPR30 in Proliferative Response of Human Bladder Cancer Cell to Estrogen. (18th May 2015)

• Record Type: Journal Article
• Authors: Huang, Weiren; Chen, Yuanbin; Liu, Yuchen; Zhang, Qiaoxia; Yu, Zhou; Mou, Lisha; Wu, Hanwei; Zhao, Li; Long, Ting; Qin, Danian; Gui, Yaoting
• Other Names: Ahluwalia Tarunveer S. Academic Editor.
• Is Part Of: BioMed research international. Volume 2015(2015)
• Publication Date: 2015-05-18
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2015/251780
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Bladder cancer belongs to one of the most common cancers and is a leading cause of deaths in our society. Urothelial carcinoma of the bladder (UCB) is the main type of this cancer, and the estrogen receptors in UCB remain to be studied. Our experiment aimed to investigate the possible biological effect of 17 β -estradiol on human bladder-derived T24 carcinoma cells and to indicate its related mechanisms. T24 cells were treated with various doses of 17 β -estradiol, and cell proliferation was detected using MTT assays. 17 β -estradiol promoted T24 cell proliferation independent of ER β /GPR30-regulated EGFR-MAPK pathway, while it inhibited cell growth via GPR30. Furthermore, the expression levels of downstream genes ( c-FOS, BCL-2, and CYCLIN D1 ) were increased by 17 β -estradiol and this effect was independently associated with activity of the EGFR-MAPK pathway. The two estrogen receptors might be potential therapeutic targets for the treatment of bladder cancer.