CpxR negatively regulates IncFII-replicon plasmid pEC011 conjugation by directly binding to multi-promoter regions. (5th December 2022)

• Record Type: Journal Article
• Authors: Jia, Yating; Hu, Huihui; Zhai, Yajun; Zhao, Bing; Sun, Huarun; Hu, Gongzheng; Pan, Yushan; Yuan, Li
• Is Part Of: Research in veterinary science. Volume 150(2022)
• Page Start: 98
• Page End: 106
• Publication Date: 2022-12-05
• Subjects: CpxR -- IncFII replicon -- Modulation -- Multidrug-resistance -- Plasmid mating; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2022.05.016
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: CpxAR is a global regulatory protein and has important roles in plasmid mating. However, except for traJ, the regulatory effect of CpxAR on other tra genes is unclear. The aim of this study was to explore the effects of CpxAR on conjugative transfer of the epidemic plasmid pEC011 (IncFII replicon) in Escherichia coli . The plasmid mating frequencies were significantly higher for the single deletion mutant strain FΔ cpxR than for the parental strain F25922. Additionally, expression levels of traM, traJ and traY in FΔ cpxR were significantly higher than those in F25922. Further investigations revealed that His6 − CpxR protein could directly bind to the traM, traJ and traY promoter regions with the binding sites of 5′-TTTACATT-3′ (PM ), 5′-ATAAGAAT-3′ (PJ ), and 5′-AATTTTAT-3′ (PY ), respectively. Taken together, our results demonstrate that CpxAR can downregulate the expression of traM, traJ and traY by directly binding to the CpxR box-like sites of promoters, thus significantly reducing the mating rates of IncFII replicon plasmid pEC011. Highlights: CpxR can directly bind to the promoters of traM, traJ and traY, and significantly down regulate their expressions, resulting in a significant decrease in the mating rate of plasmid pEC011. This study provides the theoretical basis for controlling the transfer of IncFII plasmid, which will contribute to control the spread of bla CTX-M gene.