Regulation of Angiogenic Functions by Angiopoietins through Calcium-Dependent Signaling Pathways. (4th June 2015)

• Record Type: Journal Article
• Authors: Pafumi, Irene; Favia, Annarita; Gambara, Guido; Papacci, Francesca; Ziparo, Elio; Palombi, Fioretta; Filippini, Antonio
• Other Names: Li Jie-Hui Academic Editor.
• Is Part Of: BioMed research international. Volume 2015(2015)
• Publication Date: 2015-06-04
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2015/965271
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Angiopoietins are vascular factors essential for blood vessel assembly and correct organization and maturation. This study describes a novel calcium-dependent machinery activated through Angiopoietin-1/2-Tie receptor system in HUVECs monolayer. Both cytokines were found to elicit intracellular calcium mobilization. Targeting intracellular Ca 2+ signaling, antagonizing IP3 with 2-APB or cADPR with 8Br-cADPR, was found to modulate in vitro angiogenic responses to Angiopoietins in a specific way. 2-APB and 8Br-cADPR impaired the phosphorylation of AKT and FAK induced by Ang-1 and Ang-2. On the other hand, phosphorylation of ERK1/2 and p38, as well as cell proliferation, was not affected by either inhibitor. The ability of ECs to migrate following Angs stimulation, evaluated by "scratch assay, " was reduced by either 2-APB or 8Br-cADPR following Ang-2 stimulation and only slightly affected by 2-APB in cells stimulated with Ang-1. These results identify a novel calcium-dependent machinery involved in the complex interplay regulating angiogenic processes showing that IP3 - and cADPR-induced Ca 2+ release specifically regulates distinct Angs-mediated angiogenic steps.