Effects of Chronic REM Sleep Restriction on D1 Receptor and Related Signal Pathways in Rat Prefrontal Cortex. (22nd February 2015)

• Record Type: Journal Article
• Authors: Han, Yan; Wen, Xiaosa; Rong, Fei; Chen, Xinmin; Ouyang, Ruying; Wu, Shuai; Nian, Hua; Ma, Wenling
• Other Names: Gildea John J. Academic Editor.
• Is Part Of: BioMed research international. Volume 2015(2015)
• Publication Date: 2015-02-22
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2015/978236
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : The prefrontal cortex (PFC) mediates cognitive function that is sensitive to disruption by sleep loss, and molecular mechanisms regulating neural dysfunction induced by chronic sleep restriction (CSR), particularly in the PFC, have yet to be completely understood. The aim of the present study was to investigate the effect of chronic REM sleep restriction (REM-CSR) on the D1 receptor (D1 R) and key molecules in D1 R' signal pathways in PFC. We employed the modified multiple platform method to create the REM-CSR rat model. The ultrastructure of PFC was observed by electron microscopy. HPLC was performed to measure the DA level in PFC. The expressions of genes and proteins of related molecules were assayed by real-time PCR and Western blot, respectively. The general state and morphology of PFC in rats were changed by CSR, and DA level and the expression of D1 R in PFC were markedly decreased (P < 0.01, P < 0.05 ); the expression of phosphor-PKAc α was significantly lowered in CSR rats (P < 0.05 ). The present results suggested that the alteration of neuropathology and D1 R expression in PFC may be associated with CSR induced cognitive dysfunction, and the PKA pathway of D1 R may play an important role in the impairment of advanced neural function.