The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies. (26th January 2017)

• Record Type: Journal Article
• Authors: Zhang, Pan; Lehmann, Brian D.; Shyr, Yu; Guo, Yan
• Other Names: Gerdol Marco Academic Editor.
• Is Part Of: International journal of genomics. Volume 2017(2017)
• Publication Date: 2017-01-26
• Subjects: Genomes -- Periodicals; Genomics -- Periodicals; Cytogenetics -- Periodicals; Genomics; Genome; Molecular Biology; Cytogenetics; Genomes; Genomics; Periodicals; 572.86
• DOI: 10.1155/2017/1926304
• Languages: English
• ISSNs: 2314-436X

Abstract

Abstract : High throughput genomic assays empower us to study the entire human genome in short time with reasonable cost. Formalin fixed-paraffin-embedded (FFPE) tissue processing remains the most economical approach for longitudinal tissue specimen storage. Therefore, the ability to apply high throughput genomic applications to FFPE specimens can expand clinical assays and discovery. Many studies have measured the accuracy and repeatability of data generated from FFPE specimens using high throughput genomic assays. Together, these studies demonstrate feasibility and provide crucial guidance for future studies using FFPE specimens. Here, we summarize the findings of these studies and discuss the limitations of high throughput data generated from FFPE specimens across several platforms that include microarray, high throughput sequencing, and NanoString.