MYB106 is a negative regulator and a substrate for CRL3BPM E3 ligase in regulating flowering time in Arabidopsis thaliana. Issue 6 (26th March 2021)

• Record Type: Journal Article
• Authors: Hong, Liu; Niu, Fangfang; Lin, Youshun; Wang, Shuang; Chen, Liyuan; Jiang, Liwen
• Other Names: Liu Baohui guestEditor.; Tian Zhixi guestEditor.
• Is Part Of: Journal of integrative plant biology. Volume 63:Issue 6(2021)
• Page Start: 1104
• Page End: 1119
• Publication Date: 2021-03-26
• Subjects: flowering time -- MYB transcription factor -- E3 ligase -- CUL3BPM; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.13071
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: Flowering time is crucial for successful reproduction in plants, the onset and progression of which are strictly controlled. However, flowering time is a complex and environmentally responsive history trait and the underlying mechanisms still need to be fully characterized. Post‐translational regulation of the activities of transcription factors (TFs) is a dynamic and essential mechanism for plant growth and development. CRL3 BPM E3 ligase is a CULLIN3‐based E3 ligase involved in orchestrating protein stability via the ubiquitin proteasome pathway. Our study shows that the mutation of MYB106 induced early flowering phenotype while over‐expression of MYB106 delayed Arabidopsis flowering. Transcriptome analysis of myb106 mutants reveals 257 differentially expressed genes between wild type and myb106 ‐1 mutants, including Flowering Locus T ( FT ) which is related to flowering time. Moreover, in vitro electrophoretic mobility shift assays (EMSA), in vivo chromatin immunoprecipitation quantitative polymerase chain reaction (ChIP‐qPCR) assays and dual luciferase assays demonstrate that MYB106 directly binds to the promoter of FT to suppress its expression. Furthermore, we confirm that MYB106 interacts with BPM proteins which are further identified by CRL3 BPM E3 ligases as the substrate. Taken together, we have identified MYB106 as a negative regulator in the control of flowering time and a new substrate for CRL3 BPM E3 ligases in Arabidopsis. Abstract : The Arabidopsis R2R3 MYB transcription factor MYB106 functions as a negative regulator in the control of flowering time via directly repressing the expression of FLOWERING LOCUS T, and is a substrate of CULLIN‐based E3 ligases.