Anti-inflammatory and anti-oxidative activities of lemon myrtle (Backhousia citriodora) leaf extract. (2020)

• Record Type: Journal Article
• Authors: Shim, Sun-Yup; Kim, Ji-Hyun; Kho, Kang-Hee; Lee, Mina
• Is Part Of: Toxicology reports. Volume 7(2020)
• Page Start: 277
• Page End: 281
• Publication Date: 2020
• Subjects: Lemon myrtle -- ABTS -- DPPH -- IL-6 -- TNF-α -- RAW 264.7; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2020.01.018
• Languages: English
• ISSNs: 2214-7500

Abstract

Abstract: In this present study, we examined the anti-inflammatory and anti-oxidative properties of alcoholic lemon myrtle extract (LME). The total polyphenol and flavonoid content of LME were determined as 118.77 and 14.53 mg/g extract, respectively. LME showed anti-oxidative properties, such as 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2, 2′-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. The anti-inflammatory activities of LME were investigated using the lipopolysaccharide-stimulated murine macrophage RAW 264.7 cells. Pretreatment with LME was performed at non-cytotoxic concentrations of 10–100 μg/mL. LME inhibited the production of inflammatory mediators such as nitric oxide (NO). Enzyme-linked immunosorbent assay and reverse-transcriptase polymerase chain reaction (RT-PCR) revealed that pretreatment with LME suppressed the protein expression and mRNA levels of pro-inflammatory cytokines such as interleukin IL-6, and tumor necrosis factor (TNF)-α in a concentration-dependent manner, respectively. These results suggest that LME could be used as a potential therapeutic agent having potent anti-inflammatory effects that could be used to treat inflammatory bowel disease.