Effect of polyphenol-rich cranberry extracts on cariogenic biofilm properties and microbial composition of polymicrobial biofilms. (June 2019)

• Record Type: Journal Article
• Authors: Philip, Nebu; Bandara, H.M.H.N.; Leishman, Shaneen J.; Walsh, Laurence J.
• Is Part Of: Archives of oral biology. Volume 102(2019)
• Page Start: 1
• Page End: 6
• Publication Date: 2019-06
• Subjects: Cranberry -- Microbial ecology -- Dental caries; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2019.03.026
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: Polyphenol-rich cranberry extracts disrupted cariogenic properties of saliva-derived polymicrobial biofilms. Polyphenol-rich cranberry extracts modulated a beneficial change in the microbial composition of the polymicrobial biofilms. Abstract: Objective: To investigate the effect of cranberry extracts on saliva-derived polymicrobial biofilms with regards to biofilm biomass, acidogenicity, exopolysaccharide (EPS)/microbial biovolumes, colony forming unit (CFU) counts, and the relative abundance of specific caries- and health-associated bacteria. Methods: Saliva-derived polymicrobial biofilms were grown for 96 h in a cariogenic environment and treated for 2 min every 12 h over the entire biofilm growth period with 500 μg/mL cranberry extract or vehicle control. The effect of the cranberry extract on biofilm behaviour was evaluated using different assays and its influence on key cariogenic and health-associated bacterial populations was assessed with a microarray real-time quantitative PCR method. Results: Cranberry-treated biofilms showed significant drops in biomass (38% reduction, P < 0.001), acidogenicity (44% reduction, P < 0.001), EPS/microbial biovolume ratios ( P = 0.033), and CFU counts (51% reduction, P = 0.001). Furthermore, the cranberry extracts effected a significantly lower relative abundance of caries-associated Streptococcus sobrinus (fold change 0.004, P = 0.002) and Provotella denticola (0.002, P < 0.001), and a significantly higher relative abundance of the health-associated Streptococcus sanguinis (fold change 90.715, P = 0.001). Conclusions: The cranberry extract lowered biofilm biomass, acidogenicity, EPS/microbial biovolumes, CFU counts, and modulated a beneficial microbial ecological change in saliva-derived polymicrobial biofilms.