Chitosan coated pH-responsive metal-polyphenol delivery platform for melanoma chemotherapy. (15th July 2021)

• Record Type: Journal Article
• Authors: Mu, Min; Liang, Xiaoyan; Chuan, Di; Zhao, Shasha; Yu, Wei; Fan, Rangrang; Tong, Aiping; Zhao, Na; Han, Bo; Guo, Gang
• Is Part Of: Carbohydrate polymers. Volume 264(2021)
• Publication Date: 2021-07-15
• Subjects: Melanoma -- Metal-polyphenol -- pH-responsiveness -- Chitosan -- Chemotherapy; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118000
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Acid-responsive Cab@MPN/CS NPs was prepared via ion crosslinking. The introduction of CS coating can not only protect the bioactivity of MPN, but also improve the drug loading of Cab. The zeta-potential of Cab@MPN/CS can be controlled by changing the ratio of CS and Cab@MPN. The permeability of CS promotes the penetration of Cab@MPN/CS to the deeper tumor spheroids. The delivery carrier can enhance the retention time in tumor tissues and improve antitumor efficacy of chemotherapy. Abstract: The safe and effective drug delivery system is important for cancer therapy. Here in, we first constructed a delivery system Cabazitaxel(Cab)@MPN/CS between metal-polyphenol (MPN) and chitosan (CS) to deliver Cab for melanoma therapy. The preparation process is simple, green, and controllable. After introducing CS coating, the drug loading was improved from 7.56 % to 9.28 %. Cab@MPN/CS NPs released Cab continuously under acid tumor microenvironment. The zeta potential of Cab@MPN/CS NPs could be controlled by changing the ratio of Cab@MPN and CS solutions. The positively charged Cab@MPN/CS accelerate B16F10 cell internalization. After internalized, Cab@MPN/CS NPs could escape from lysosomes via the proton sponge effect. The permeability of CS promotes the penetration of Cab@MPN/CS to the deeper B16F10 tumor spheroids. In vivo results showed that Cab@MPN/CS NPs have a longer retention time in tumor tissues and significantly inhibit tumor growth by up-regulating TUNEL expression and down-regulating KI67 and CD31 expression. Thus, this delivery system provides a promising strategy for the tumor therapy in clinic.