Octanoyl glycol chitosan enhances the proliferation and differentiation of tonsil-derived mesenchymal stem cells. (15th July 2021)

• Record Type: Journal Article
• Authors: Lee, Kyeong Eun; Choi, Da Hyeon; Joo, Chanyang; Kang, Sun-Woong; Huh, Kang Moo; Park, Yoon Shin
• Is Part Of: Carbohydrate polymers. Volume 264(2021)
• Publication Date: 2021-07-15
• Subjects: bFGF basic fibroblast growth factor -- CDK cyclin-dependent kinase -- DMEM Dulbecco's modified eagle medium -- DPBS Dulbecco's phosphate-buffered saline -- FBS fetal bovine serum -- FCCP trifluoremethoxyphenylhydrazone -- FTIR fourier-transform infrared spectroscopy -- HGC hexanoyl glycol chitosan -- MWCO molecular weight cut-off -- NMR nuclear magnetic resonance -- OGC octanoyl glycol chitosan -- OCR oxygen consumption rate -- R+A rotenone and antimycin -- RFU relative fluoresceence unit -- SRC spare respiratory capacity -- TMSCs tonsil-derived mesenchymal stem cells; Octanoyl glycol chitosan (OGC) -- Cyclin D1 -- Tonsil-derived mesenchymal stem cells (TMSCs) -- Cell cycle -- Oxygen consumption ratio (OCR); Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.117992
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: OGC is a novel glycol chitosan derivative with improved water-solubility. OGC provides optimal cell-to-cell interaction for cellular activities. OGC improves cell proliferation and differentiation capacity of tonsil-derived MSCs. OGC promotes mitochondrial function of tonsil-derived MSCs to increase the Cyclin D1 protein. OGC enhances osteogenic differentiation potential of tonsil-derived MSCs. Abstract: Biofunctional polymers have been widely used to enhance the proliferation and functionality of stem cells. Here, we report the development of a new biofunctional polymer, octanoyl glycol chitosan (OGC), and demonstrate its effects on the cell cycle and stem cell function using tonsil-derived mesenchymal stem cells (TMSCs). OGC treatment (100 μg/mL) significantly increased the proliferation of TMSCs, which could be attributed to cyclin D1 up-regulation in the G1 phase of the cell cycle. Additionally, OGC enhanced the ability of TMSCs to differentiate into adipocytes, chondrocytes, and osteoblasts. Taken together, this new biofunctional polymer, OGC, can promote stemness and osteogenesis, as well as induce stem cell proliferation by enhancing the intracellular metabolic rate and regulating the cell cycle. Thus, in the future, OGC could be a potential therapeutic additive for improving stem cell function.