Characterization of hemicellulose in Cassava (Manihot esculenta Crantz) stem during xylogenesis. (15th July 2021)

• Record Type: Journal Article
• Authors: Yang, Haoqiang; Yi, Na; Zhao, Shuai; Xiang, Zhouyang; Qaseem, Mirza Faisal; Zheng, Biao; Li, Huiling; Feng, Jia-Xun; Wu, Ai-Min
• Is Part Of: Carbohydrate polymers. Volume 264(2021)
• Publication Date: 2021-07-15
• Subjects: Cassava -- Xylogenesis -- Acetyl -- Structural characterization -- Molecular weight -- Hemicellulose synthesis; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118038
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Hemicellulose in cassava is mainly composed of methylated glucuronic acid arabinoxylan. Hemicellulose in cassava plant contains tetrasaccharide reducing end sequence. The degree of hemicellulose acetylation increases with xylogenesis. The molecular weight of cassava hemicellulose also increases during xylogenesis. Hemicellulose biosynthesis in old cassava is mainly concentrated in upper stem. Abstract: Cassava is one of the three major potato crops due to the high starch content in its tubers. Unlike most current studies on the utilization of cassava tubers, our research is mainly focused on the stem of cassava plant. Through nuclear magnetic resonance (NMR), fourier transform infrared spectrometer (FTIR) and other methods, we found that cassava stalk hemicellulose consists of β-1, 4 glycosidic bond-linked xylan backbone with a tetrasaccharide reducing end and decorated with methylated glucuronic acid, acetyl groups and a high degree of arabinose substitutions. Hemicellulose content gradually increased from the upper to the lower parts of the stem. The apical part of cassava stalk contained more branched and heterogeneous glycans than the middle and basal parts, and the molecular weight of hemicellulose increased from top to bottom. Our findings will be helpful in understanding of structural variations of cassava hemicellulose during xylogenesis, as well as in better utilization of cassava plant waste in industry.