The in vitro assessment of a novel vaping technology. (2020)

• Record Type: Journal Article
• Authors: Breheny, D.; Thorne, D.; Baxter, A.; Bozhilova, S.; Jaunky, T.; Santopietro, S.; Taylor, M.; Terry, A.; Gaça, M.
• Is Part Of: Toxicology reports. Volume 7(2020)
• Page Start: 1145
• Page End: 1156
• Publication Date: 2020
• Subjects: ACM aerosol collected mass -- AqE aqueous aerosol extracts -- ALI air-liquid interface -- ANOVA analysis of variance -- ARE antioxidant response element -- AqE aerosol aqueous extract -- CRM81 CORESTA recommended method number 81 -- DCF 2′, 7′ dichlorodihydrofluorescein -- DMSO dimethyl sulfoxide -- DSB double-strand break -- FDA US Food and Drug Administration -- GEF global evaluation factor -- GSH glutathione (reduced form) -- HCI Health Canada Intense -- HUVEC human umbilical vein endothelial cell -- ISO International Organisation for Standardisation -- IVMn in vitro micronucleus -- MF mutant frequency -- MLA mouse lymphoma assay -- NASEM US National Academy of Sciences, Engineering and Medicine -- NHBE normal human bronchial epithelial -- NRU neutral red uptake -- NVP new vapour product -- RWD relative wound density -- S9 post-mitochondrial supernatant -- TobReg WHO Study Group on Tobacco Product Regulation -- TPA 12-O-tetradecanoylphorbol-13-acetate -- TPM total particulate matter -- WA whole aerosol; Cigarette -- Electronic cigarette -- In vitro -- Aerosol; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2020.08.016
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Abstract: We have developed a novel vaping product (NVP) IS1.0(TT), which utilises a stainless-steel mesh to transfer and vaporise the e-liquid, mitigating some of the potential sources of toxicants that can be generated using the more traditional 'wick and coil' approach. The emissions from IS1.0(TT) have previously been found to have lower levels of toxicants overall when directly compared with a commercial wick and coil e-cig. This current study assessed the toxicological responses to aerosols from this NVP. Responses induced by IS1.0(TT)were compared to those from a 3R4F reference cigarette, using in vitro test methods which included regulatory genetic toxicological assays as well as some more contemporary screening approaches. The experimental conditions were designed to facilitate the testing of aerosol from this vaping product at doses that in most cases greatly exceeded those of the 3R4F comparator showed little to no toxicological responses and demonstrated significantly reduced effects in these in vitro assays when compared to 3R4F. Furthermore, the extreme doses tested in the present study indicate that the toxicant profile of this NVP translates to lower biological activity in vitro, and suggests that the absolute risk hazard level associated with electronic cigarettes can be reduced through continuous improvement as the technology evolves.