Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus. (1st December 2017)

• Record Type: Journal Article
• Authors: Lyoo, Kwang‐Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
• Is Part Of: Veterinary record. Volume 181:Number 22(2017)
• Page Start: 596
• Page End: 601
• Publication Date: 2017-12-01
• Subjects: porcine epidemic diarrhea virus -- immunochromatographic test kit -- rapid diagnosis; Veterinary medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Veterinary medicine; Veterinary Medicine; Periodicals; Periodical; Electronic journals; 636.089
• DOI: 10.1136/vr.103959
• Languages: English
• ISSNs: 0042-4900

Abstract

Abstract : Porcine epidemic diarrhoea virus (PEDV) causes acute and severe watery diarrhoea and dehydration, as well as 50–100 per cent mortality in piglets. For the PEDV diagnosis, a rapid test kit that is specific and sensitive to PEDV is critical to monitor this disease at pig farms. The present study aimed to develop an immunochromatographic assay (ICA) strip test for detecting PEDV in faecal swabs. The newly developed diagnostic test showed a detection limit of 10 4.0 TCID50 /ml of PEDV. Using faecal swab samples, the relative sensitivity and specificity of the ICA kit were 95.0 per cent and 98.6 per cent, respectively, compared with those of real‐time RT‐PCR. In samples from piglets experimentally infected with PEDV, the results showed 100 per cent agreement with those found by real‐time RT‐PCR. Our developed test strip will be useful for rapid diagnosis and can be used for epidemiological surveillance of PEDV infection.