Effects of TLR3 and TLR9 Signaling Pathway on Brain Protection in Rats Undergoing Sevoflurane Pretreatment during Cardiopulmonary Bypass. (27th December 2017)

• Record Type: Journal Article
• Authors: Nan, Zhou; Jin, Zhou; Huijuan, Cao; Tiezheng, Zhang; Keyan, Chen
• Other Names: Conti Alfredo Academic Editor.
• Is Part Of: BioMed research international. Volume 2017(2017)
• Publication Date: 2017-12-27
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2017/4286738
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Objective . To investigate the effects of TLR3 and TLR9 signaling pathway on brain injury during CPB in rats pretreated with sevoflurane and its possible molecular mechanism. Methods . SD rats were randomly assigned to sham group, CPB group, and Sev group. Brain tissue was obtained at before CPB (T 0 ), at CPB for 30 minutes (T 1 ), 1 hour after CPB (T 3 ), and 3 hours after CPB (T 5 ). ELISA was used to measure S100- β and IL-6. Western blot was utilized to determine TLR3 and TLR9 expression. TUNEL was applied to detect neuronal apoptosis. Results . Compared with CPB group, at T 1, at termination after 1 hour of CPB (T 2 ), T 3, 2 hours after CPB (T 4 ) and T 5, S100- β and IL-6 decreased in Sev group. Compared with CPB group, IFN- β were increased in Sev group, except T 0 . Compared with CPB group, TLR3 expression increased, and TLR9 and NF- κ B decreased in Sev group. The apoptotic neurons were less in Sev group than in CPB group (P < 0.05 ). Conclusion . Sevoflurane intervention can activate TLR3 and TLR9 signaling pathway, upregulate TLR3 expression and downstream TRIF expression, decrease TLR9 expression, and downregulate downstream NF- κ B expression in CPB rat models, thereby mitigating brain injury induced by inflammatory response during CPB.