Protein Array-Based Detection of Proteins in Kidney Tissues from Patients with Membranous Nephropathy. (27th February 2017)

• Record Type: Journal Article
• Authors: Wang, Shuqiang; Lu, Yang; Hong, Quan; Geng, Xiaodong; Wang, Xu; Zheng, Wei; Song, Chengcheng; Liu, Chunling; Fan, Meng; Xi, Yue; Guo, Mandi; Wu, Di
• Other Names: Liu Gang Academic Editor.
• Is Part Of: BioMed research international. Volume 2017(2017)
• Publication Date: 2017-02-27
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2017/7843584
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Membranous nephropathy (MN) is an autoimmune inflammatory disease in which proteins related with plenty of biological processes play an important role. However, the role of these proteins in the pathogenesis of MN is still unclear. This study aimed to screen differential proteins in kidney tissue samples from MN patients by using protein arrays and determine the pathways involved in the pathogenesis of MN. This study first tested a quantitative protein array (QAH-INF-3) and two semiquantitative protein arrays (L-493 and L-507) with normal renal tissue and identified L-493 as the most appropriate assay to compare protein levels between MN tissues and normal control tissues. The L-493 array identified 66 differentially expressed proteins (DEPs) that may be associated with MN. The gene oncology (GO) and protein-protein interaction (PPI) analyses revealed several processes potentially involved in MN, including extracellular matrix disassembly and organization, cell adhesion, cell-cell signaling, cellular protein metabolic process, and immune response (P < 0.0 5 ). We suggest that these different pathways work together via protein signaling and result in the pathogenesis and progression of MN.