Non-human Primate Total-body Irradiation Model with Limited and Full Medical Supportive Care Including Filgrastim for Biodosimetry and Injury Assessment. Issue 1 (23rd December 2016)

• Record Type: Journal Article
• Authors: Ossetrova, Natalia I.; Blakely, William F.; Nagy, Vitaly; McGann, Camille; Ney, Patrick H.; Christensen, Christine L.; Koch, Amory L.; Gulani, Jatinder; Sigal, George B.; Glezer, Eli N.; Hieber, Kevin P.
• Is Part Of: Radiation protection dosimetry. Volume 172:Issue 1/3(2016)
• Page Start: 174
• Page End: 191
• Publication Date: 2016-12-23
• Subjects: Radiation dosimetry -- Periodicals; Radiation -- Safety measures -- Periodicals; 363.1799
• DOI: 10.1093/rpd/ncw176
• Languages: English
• ISSNs: 0144-8420

Abstract

Abstract: An assessment of multiple biomarkers from radiation casualties undergoing limited- or full-supportive care including treatment with filgrastim is critical to develop rapid and effective diagnostic triage strategies. The efficacy of filgrastim with full-supportive care was compared with results with limited-supportive care by analyzing survival, necropsy, histopathology and serial blood samples for hematological, serum chemistry and protein profiles in a non-human primate ( Macaca mulatta, male and female) model during 60-d post-monitoring period following sham- and total-body irradiation with 6.5 Gy 60 Co gamma-rays at 0.6 Gy min −1 . Filgrastim (10 μg kg −1 ) was administered beginning on Day 1 post-exposure and continued daily until neutrophil counts were ≥2, 000 μL −1 for two consecutive days. Filgrastim and full-supportive care significantly decreased the pancytopenia duration and resulted in improved animal survival and recovery compared to animals with a limited-supportive care. These findings also identified and validated a multiparametric biomarker panel to support radiation diagnostic device development.