The Clinicopathological Significance of miR-133a in Colorectal Cancer. (30th June 2014)

• Record Type: Journal Article
• Authors: Wan, Timothy Ming-Hun; Lam, Colin Siu-Chi; Ng, Lui; Chow, Ariel Ka-Man; Wong, Sunny Kit-Man; Li, Hung-Sing; Man, Johnny Hon-Wai; Lo, Oswens Siu-Hung; Foo, Dominic; Cheung, Alvin; Yau, Thomas; Poon, Jensen Tung-Chung; Poon, Ronnie Tung-Ping; Law, Wai-Lun; Pang, Roberta Wen-Chi
• Other Names: Huang Yi-Chia Academic Editor.
• Is Part Of: Disease markers. Volume 2014(2014)
• Publication Date: 2014-06-30
• Subjects: Diagnosis -- Periodicals; Biochemical markers -- Periodicals; Pathology -- Periodicals; 616
• DOI: 10.1155/2014/919283
• Languages: English
• ISSNs: 0278-0240

Abstract

Abstract : This study determined the expression of microRNA-133a (MiR-133a) in colorectal cancer (CRC) and adjacent normal mucosa samples and evaluated its clinicopathological role in CRC. The expression of miR-133a in 125 pairs of tissue samples was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and correlated with patient's clinicopathological data by statistical analysis. Endogenous expression levels of several potential target genes were determined by qRT-PCR and correlated using Pearson's method. MiR-133a was downregulated in 83.2% of tumors compared to normal mucosal tissue. Higher miR-133a expression in tumor tissues was associated with development of distant metastasis, advanced Dukes and TNM staging, and poor survival. The unfavorable prognosis of higher miR-133a expression was accompanied by dysregulation of potential miR-133a target genes, LIM and SH3 domain protein 1 (LASP1), Caveolin-1 (CAV1), and Fascin-1 (FSCN1). LASP1 was found to possess a negative correlation (γ = - 0.23 ), whereas CAV1 exhibited a significant positive correlation (γ = 0.27 ), and a stronger correlation was found in patients who developed distant metastases (γ = 0.42 ). In addition, a negative correlation of FSCN1 was only found in nonmetastatic patients. In conclusion, miR-133a was downregulated in CRC tissues, but its higher expression correlated with adverse clinical characteristics and poor prognosis.