MiR‐143‐3p suppresses cell proliferation, migration, and invasion by targeting Melanoma‐Associated Antigen A9 in laryngeal squamous cell carcinoma. Issue 2 (9th October 2018)

• Record Type: Journal Article
• Authors: Han, Liang; Tang, Mingming; Xu, Xinjiang; Jiang, Bin; Wei, Yingze; Qian, Hongyan; Lu, Xueguan
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 2(2019)
• Page Start: 1245
• Page End: 1257
• Publication Date: 2018-10-09
• Subjects: invasion -- laryngeal squamous cell carcinoma (LSCC) -- melanoma‐associated antigens‐A9 (MAGE‐A9) -- migration -- miR‐143‐3p -- proliferation; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.27084
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Previously we found that melanoma‐associated antigen‐A9 (MAGE‐A9) was a significantly upregulated biomarker in laryngeal squamous cell carcinoma (LSCC). A high expression of MAGE‐A9 indicates an unfavorable survival outcome, and the MAGE‐A9 expression level is an independent prognostic factor of LSCC. To explore the mechanism of MAGE‐A9 upregulation, several predicted regulatory microRNAs were screened and validated in LSCC cells. In the current study, we found that miR‐143‐3p (MAGE‐A9 related miRNAs) expression levels correlated negatively with the MAGE‐A9 protein expression in LSCC tissues. Dual‐luciferase reporter assays and Western blot analysis revealed MAGE‐A9 to be a direct target of miR‐143‐3p. Furthermore, a series of in vitro gain‐ and loss‐of‐function assays revealed that miR‐143‐3p inhibited LSCC cell proliferation, migration, and invasion. Also, miR‐143‐3p suppressed LSCC tumorigenesis in vivo. These effects were clinically relevant, as a lower expression of miR‐143‐3p occurred in severer clinical stages and represented poor overall survival in patients with LSCC. Taken together, these results suggest that downregulation of miR‐143‐3p contributes to tumor progression through upregulation of MAGE‐A9. The expression level of these two key molecules maintained LSCC progression, thus, highlighting the potential of miR‐143‐3p as a therapeutic target for human LSCC. Abstract : MiR‐143‐3p is downregulated and melanoma antigen‐A9 (MAGE‐A9) is upregulated in laryngeal squamous cell carcinoma (LSCC) tissues. MAGE‐A9 is a direct target of miR‐143‐3p. miR‐143‐3p suppresses cell proliferation, migration, and invasion in LSCC.