Characterization of Myelomonocytoid Progenitor Cells with Mesenchymal Differentiation Potential Obtained by Outgrowth from Pancreas Explants. (21st August 2012)

• Record Type: Journal Article
• Authors: Roehrich, Marc-Estienne; Vassalli, Giuseppe
• Other Names: Monteiro Gabriel A. Academic Editor.
• Is Part Of: Biotechnology research international. Volume 2012(2012)
• Publication Date: 2012-08-21
• Subjects: Biotechnology -- Periodicals; Biotechnology; Biotechnology; Periodicals; 660.6
• DOI: 10.1155/2012/429868
• Languages: English
• ISSNs: 2090-3138

Abstract

Abstract : Progenitor cells can be obtained by outgrowth from tissue explants during primary ex vivo tissue culture. We have isolated and characterized cells outgrown from neonatal mouse pancreatic explants. A relatively uniform population of cells showing a distinctive morphology emerged over time in culture. This population expressed monocyte/macrophage and hematopoietic markers (CD11b + and CD45 + ), and some stromal-related markers (CD44 + and CD29 + ), but not mesenchymal stem cell (MSC)-defining markers (CD90 − and CD105 − ) nor endothelial (CD31 − ) or stem cell-associated markers (CD133 − and stem cell antigen-1; Sca-1 − ). Cells could be maintained in culture as a plastic-adherent monolayer in culture medium (MesenCult MSC) for more than 1 year. Cells spontaneously formed sphere clusters "pancreatospheres" which, however, were nonclonal. When cultured in appropriate media, cells differentiated into multiple mesenchymal lineages (fat, cartilage, and bone). Positive dithizone staining suggested that a subset of cells differentiated into insulin-producing cells. However, further studies are needed to characterize the endocrine potential of these cells. These findings indicate that a myelomonocytoid population from pancreatic explant outgrowths has mesenchymal differentiation potential. These results are in line with recent data onmonocyte-derivedmesenchymal progenitors (MOMPs).