Effect of a Silver Nanoparticles Solution on Staphylococcus aureus and Candida spp. (19th August 2014)

• Record Type: Journal Article
• Authors: Wady, Amanda Fucci; Machado, Ana Lucia; Foggi, Camila Cristina; Zamperini, Camila Andrade; Zucolotto, Valtencir; Moffa, Eduardo Buozi; Vergani, Carlos Eduardo
• Other Names: Correa-Duarte Miguel A. Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2014(2014)
• Publication Date: 2014-08-19
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2014/545279
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : An AgNPs solution was synthesized by chemical reduction, characterized, and tested against Candida glabrata, Candida tropicalis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA). Minimum inhibitory (MICs) and minimum fungicidal/bactericidal concentrations (MFC/MBC) were determined on planktonic cells. Also, total biofilm mass was determined by crystal violet (CV) staining and morphological changes by scanning electron microscope (SEM). MICs for C. glabrata, C. tropicalis, S. aureus, and MRSA were 15.63, 3.91, 1.95, and 1.95 µ g/mL, respectively. MFC for C. glabrata was 62.5 µ g/mL and for C. tropicalis 15.63 µ g/mL The same MBC (3.91 µ g/mL) was observed for S. aureus and MRSA. CV assay showed that the AgNPs (1000 μ g/mL) promoted reductions in biofilm mass of ~60% for C. glabrata and ~35% for C. tropicalis . A reduction of ~20% in C. tropicalis biomass was also observed at the concentration of 3.91 µ g/mL. No significant effect on total biomass was found for S. aureus and MRSA. SEM images revealed that C. glabrata and C. tropicalis biofilm cells, exposed to the AgNPs (1000 μ g/mL), had an irregular and shriveled appearance. AgNPs solution exhibited considerable antimicrobial activity against important fungal and bacterial pathogens, associated with several oral and systemic diseases, and has potential as an antimicrobial agent.