Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice. (22nd December 2016)

• Record Type: Journal Article
• Authors: Fu, Xiaolong; Zhang, Linqing; Jin, Yecheng; Sun, Xiaoyang; Zhang, Aizhen; Wen, Zongzhuang; Zhou, Yichen; Xia, Ming; Gao, Jiangang
• Other Names: Wang Jian Academic Editor.
• Is Part Of: Neural plasticity. Volume 2016(2016)
• Publication Date: 2016-12-22
• Subjects: Neuroplasticity -- Periodicals; 612.82
• DOI: 10.1155/2016/6720420
• Languages: English
• ISSNs: 2090-5904

Abstract

Abstract : MYH14 is a member of the myosin family, which has been implicated in many motile processes such as ion-channel gating, organelle translocation, and the cytoskeleton rearrangement. Mutations in MYH14 lead to a DFNA4-type hearing impairment. Further evidence also shows that MYH14 is a candidate noise-induced hearing loss (NIHL) susceptible gene. However, the specific roles of MYH14 in auditory function and NIHL are not fully understood. In the present study, we used CRISPR/Cas9 technology to establish a Myh14 knockout mice line in CBA/CaJ background (now referred to as Myh14 −/− mice) and clarify the role of MYH14 in the cochlea and NIHL. We found that Myh14 −/− mice did not exhibit significant hearing loss until five months of age. In addition, Myh14 −/− mice were more vulnerable to high intensity noise compared to control mice. More significant outer hair cell loss was observed in Myh14 −/− mice than in wild type controls after acoustic trauma. Our findings suggest that Myh14 may play a beneficial role in the protection of the cochlea after acoustic overstimulation in CBA/CaJ mice.