TLR4 Signaling in MPP+-Induced Activation of BV-2 Cells. (5th January 2016)

• Record Type: Journal Article
• Authors: Zhou, Peng; Weng, Ruihui; Chen, Zhaoyu; Wang, Rui; Zou, Jing; Liu, Xu; Liao, Jinchi; Wang, Yanping; Xia, Ying; Wang, Qing
• Other Names: Kaur Charanjit Academic Editor.
• Is Part Of: Neural plasticity. Volume 2016(2016)
• Publication Date: 2016-01-05
• Subjects: Neuroplasticity -- Periodicals; 612.82
• DOI: 10.1155/2016/5076740
• Languages: English
• ISSNs: 2090-5904

Abstract

Abstract : Aims. This work was conducted to establish an in vitro Parkinson's disease (PD) model by exposing BV-2 cells to 1-methyl-4-phenylpyridinium (MPP + ) and exploring the roles of TLR2/TLR4/TLR9 in inflammatory responses to MPP + . Methods/Results. MTT assay showed that cell viability of BV-2 cells was 84.78 ± 0.86% and 81.18 ± 0.99% of the control after incubation with 0.1 mM MPP + for 12 hours and 24 hours, respectively. Viability was not significantly different from the control group. With immunofluorescence technique, we found that MPP + incubation at 0.1 mM for 12 hours was the best condition to activate BV-2 cells. In this condition, the levels of TNF- α, IL-1 β, and iNOS protein were statistically increased compared to the control according to ELISA tests. Real time RT-PCR and western blot measurements showed that TLR4 was statistically increased after 0.1 mM MPP + incubation for 12 hours. Furthermore, after siRNA interference of TLR4 mRNA, NF- κ B activation and the levels of TNF- α, IL-1 β, and iNOS were all statistically decreased in this cell model. Conclusion. MPP + incubation at the concentration of 0.1 mM for 12 hours is the best condition to activate BV-2 cells for mimicking PD inflammation in BV-2 cells. TLR4 signalling plays a critical role in the activation of BV-2 cells and the induction of inflammation in this cell model.