Positive Correlation between IP-10 and IFN-γ Levels in Rhesus Monkeys (Macaca mulatta) with Either Naturally Acquired or Experimental Infection of Mycobacterium tuberculosis. (20th April 2017)

• Record Type: Journal Article
• Authors: Min, Fangui; Wu, Ruike; Pan, Jinchun; Huang, Shuwu; Luo, Yinzhu; Zhang, Yu
• Other Names: Goldmann Torsten Academic Editor.
• Is Part Of: BioMed research international. Volume 2017(2017)
• Publication Date: 2017-04-20
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2017/5089752
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Numerous studies identify that IP-10 and IFN- γ are involved in leucocyte migration and activation and regarded as promising surrogate biomarkers in human and bovine tuberculosis infection, but there is lack of evidence for IP-10 in nonhuman primates. In this study, we directly determined IP-10 and IFN- γ levels in plasma from 30 healthy monkeys, 30 monkeys with naturally acquired tuberculosis, 4 monkeys experimentally infected with tuberculosis, and PPD stimulated whole blood of 14 monkeys with naturally acquired tuberculosis by ELISA. Higher plasma levels of IP-10 and IFN- γ were observed in natural tuberculosis monkeys than in healthy controls. The dynamic changes of plasma IP-10 and IFN- γ in experimental infections showed consistent representation of a transient increase during the infection period. After PPD stimulation, release of IP-10 and IFN- γ is significantly induced in natural tuberculosis monkeys, but the stimulation index of IP-10 was significantly lower than IFN- γ . Further analysis showed that positive correlation between IP-10 and IFN- γ existed in healthy and tuberculosis monkeys. Our findings support plasma IP-10 and IFN- γ as biomarkers for monitoring ongoing inflammation of nonhuman primate tuberculosis, and IFN- γ is a more valuable diagnostic biomarker.