Laccase production by Pleurotus ostreatus using cassava waste and its application in remediation of phenolic and polycyclic aromatic hydrocarbon-contaminated lignocellulosic biorefinery wastewater. (15th September 2022)

• Record Type: Journal Article
• Authors: Kumar, Vaidyanathan Vinoth; Venkataraman, Swethaa; Kumar, P. Senthil; George, Jenet; Rajendran, Devi Sri; Shaji, Anna; Lawrence, Nicole; Saikia, Kongkona; Rathankumar, Abiram Karanam
• Is Part Of: Environmental pollution. Volume 309(2022)
• Publication Date: 2022-09-15
• Subjects: Pleurotus ostreatus -- Polycyclic aromatic hydrocarbons -- Laccase -- Lignocellulosic biorefinery wastewater -- Organic contaminants -- Cassava waste; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.119729
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The treatment of contaminants from lignocellulosic biorefinery effluent has recently been identified as a unique challenge. This study focuses on removing phenolic contaminants and polycyclic aromatic hydrocarbons (PAHs) from lignocellulosic biorefinery wastewater (BRW) applying a laccase-assisted approach. Cassava waste was used as a substrate to produce the maximum yield of laccase enzyme (3.9 U/g) from Pleurotus ostreatus . Among the different inducers supplemented, CuSO4 (0.5 mM) showed an eight-fold increase in enzyme production (30.8 U/g) after 240 h of incubation. The catalytic efficiency of laccase was observed as 128.7 ± 8.47 S −1 mM −1 for syringaldazine oxidation at optimum pH 4.0 and 40 °C. Laccase activity was completely inhibited by lead (II) ion, mercury (II) ion, sodium dodecyl sulphate, sodium azide and 1, 4 dithiothretiol and induced significantly by manganese (II) ion and rhamnolipid. After treating BRW with laccase, the concentrations of PAHs and phenolic contaminants of 1144 μg/L and 46160 μg/L were reduced to 96 μg/L and 16100 μg/L, respectively. The ability of laccase to effectively degrade PAHs in the presence of different phenolic compounds implies that phenolic contaminants may play a role in PAHs degradation. After 240 h, organic contaminants were removed from BRW in the following order: phenol >2, 4-dinitrophenol > 2-methyl-4, 6-dinitrophenol > 2, 3, 4, 6-tetrachlorophenol > acenaphthene > fluorine > phenanthrene > fluoranthene > pyrene > anthracene > chrysene > naphthalene > benzo(a)anthracene > benzo(a)pyrene > benzo(b)fluoranthene > pentachlorophenol > indeno(1, 2, 3-cd)pyrene > benzo(j) fluoranthene > benzo[k]fluoranthène. The multiple contaminant remediation from the BRW by enzymatic method, clearly suggests that the laccase can be used as a bioremediation tool for the treatment of wastewater from various industries. Graphical abstract: Image 1 Highlights: Pleurotus ostreatus was grown on starch-based industry waste to produce laccase. After 240 h of fermentation, 0.5 mM CuSO4 induced 30 U/g of laccase production. Wastewater exhibits the presence of 15 PAHs (1.1 mg/L) and 5 phenols (46.3 mg/L). 91% of PAHs and 65% of phenolic compounds were remediated by laccase in wastewater. Low molecular weight PAHs have higher remediation rates by enzymatic treatment.