Regulation of Proinflammatory Mediators via NF-κB and p38 MAPK-Dependent Mechanisms in RAW 264.7 Macrophages by Polyphenol Components Isolated from Korea Lonicera japonica THUNB. (7th May 2012)

• Record Type: Journal Article
• Authors: Park, Kwang-Il; Kang, Sang-Rim; Park, Hyeon-Soo; Lee, Do Hoon; Nagappan, Arulkumar; Kim, Jin A; Shin, Sung Chul; Kim, Eun Hee; Lee, Won Sup; Chung, Hyon-Jong; An, Su Jin; Kim, Gon Sup
• Other Names: Saad Bashar Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2012(2012)
• Publication Date: 2012-05-07
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2012/828521
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Lonicera japonica THUNB., which abundantly contains polyphenols, has been used as a traditional medicine for thousands of years in East Asian countries because of the anti-inflammation properties. This study aimed to investigate the anti-inflammatory mechanism of polyphenol components isolated from Korea L. japonica T. by nuclear factor-kappaB (NF- κ B) and mitogen-activated protein kinases (MAPKs) pathway. Polyphenols significantly decreased lipopolysaccharide- (LPS-) induced mRNA and protein expression of inducible nitric oxide synthase and cyclooxygenase-2, as well as mRNA expression of tumor necrosis factor-alpha, interleukin- (IL-) 1 β, and IL-6. Moreover, polyphenols inhibited nuclear translocation of NF- κ B p65, phosphorylation/degradation of the inhibitor of κ B, and phosphorylation of p38 MAPK, whereas the extracellular signal-regulated kinase and Janus N-terminal kinase were not affected. These results indicate that polyphenol components isolated from Korea L. japonica T. should have anti-inflammatory effect on LPS-stimulated RAW 264.7 cells through the decrease of proinflammatory mediators expression by suppressing NF- κ B and p38 MAPK activity.