Blood and Lymph Node Dissemination of Clonal Genome-Intact Human Immunodeficiency Virus 1 DNA Sequences During Suppressive Antiretroviral Therapy. (1st April 2020)

• Record Type: Journal Article
• Authors: Kuo, Hsiao-Hsuan; Banga, Riddhima; Lee, Guinevere Q; Gao, Ce; Cavassini, Matthias; Corpataux, Jean-Marc; Blackmer, Jane E; zur Wiesch, Schulze; Yu, Xu G; Pantaleo, Giuseppe; Perreau, Matthieu; Lichterfeld, Mathias
• Is Part Of: Journal of infectious diseases. Volume 222:Number 4(2020)
• Page Start: 655
• Page End: 660
• Publication Date: 2020-04-01
• Subjects: HIV-1 -- reservoir -- Lymph node -- proviral sequencing -- CD4 T cells; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa137
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: The majority of cells with latent human immunodeficiency virus 1 infection are located in lymphoid tissues that are difficult to access. In the current study, we used single-genome near-full-length proviral sequencing to evaluate intact and defective proviruses in blood and lymph node CD4 T cells enriched for specific functional polarizations. We observed minor variations between the frequencies of proviral sequences within individual CD4 T-cell subsets and across tissue compartments. However, we noted multiple clonal clusters of identical intact or defective proviral sequences from distinct compartments and CD4 T-cell subpopulations, suggesting frequent interchanges between viral reservoir cells in blood and tissues. Abstract : Using single-genome, near-full-length viral sequencing, we showed that clonal clusters of intact and defective human immunodeficiency virus 1 DNA sequences from patients receiving antiretroviral treatment patients consist of mixed proviral species from blood and lymph nodes.