Cigarette smoking is associated with intestinal barrier dysfunction in the small intestine but not in the large intestine of mice. (1st December 2014)

• Record Type: Journal Article
• Authors: Zuo, Lugen; Li, Yi; Wang, Honggang; Wu, Rong; Zhu, Weiming; Zhang, Wei; Cao, Lei; Gu, Lili; Gong, Jianfeng; Li, Ning; Li, Jieshou
• Is Part Of: Journal of Crohn's and colitis. Volume 8:Number 12(2014)
• Page Start: 1710
• Page End: 1722
• Publication Date: 2014-12-01
• Subjects: Cigarette smoke -- Intestinal barrier -- Animal model -- Crohn's disease -- Ulcerative colitis; Inflammatory bowel diseases -- Periodicals; 616.344005
• DOI: 10.1016/j.crohns.2014.08.008
• Languages: English
• ISSNs: 1873-9946

Abstract

Abstract: Aims: To observe the effect of cigarette smoke (CS) on the small bowel and colon in mice and to attempt to explain the potential mechanisms that account for these effects. Methods: Male BALB/c mice age 6–8 weeks were randomly divided into a CS group and a control group (n = 10 per group). CS mice were exposed to CS (five cigarettes each time, four times a day for 5 days a week using Hamburg II smoking machine and CS was diluted with air at a ratio of 1:6) for 10 weeks, and control mice were exposed to room air. After 10 weeks, mice were sacrificed for analysis (colon and small bowel). Results: CS exposure impaired the intestinal barrier of the small bowel, based on evidence that CS mice exhibited increased intestinal permeability, bacterial translocation, intestinal villi atrophy, damaged tight junctions and abnormal tight junction proteins. These changes were partly mediated through the activated NF-κB (p65) signalling pathway. However, no obvious changes associated with the intestinal barrier were identified in the small bowel of control mice or the colons of control or CS mice. Conclusions: CS is associated with intestinal barrier dysfunction in the small intestine but not in the large intestine of mice.