Paper spray screening and liquid chromatography/mass spectrometry confirmation for medication adherence testing: A two‐step process. (31st October 2019)

• Record Type: Journal Article
• Authors: Costa, Catia; Frampas, Cecile; Longman, Katherine A.; Palitsin, Vladimir; Ismail, Mahado; Sears, Patrick; Nilforooshan, Ramin; Bailey, Melanie J.
• Is Part Of: Rapid communications in mass spectrometry. Volume 35(2021)Supplement 2
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-10-31
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/rcm.8553
• Languages: English
• ISSNs: 0951-4198

Abstract

Abstract : Rationale: Paper spray offers a rapid screening test without the need for sample preparation. The incomplete extraction of paper spray allows for further testing using more robust, selective and sensitive techniques such as liquid chromatography/mass spectrometry (LC/MS). Here we develop a two‐step process of paper spray followed by LC/MS to (1) rapidly screen a large number of samples and (2) confirm any disputed results. This demonstrates the applicability for testing medication adherence from a fingerprint. Methods: Following paper spray analysis, drugs of abuse samples were analysed using LC/MS. All analyses were completed using a Q Exactive™ Plus Orbitrap™ mass spectrometer. This two‐step procedure was applied to fingerprints collected from patients on a maintained dose of the antipsychotic drug quetiapine. Results: The extraction efficiency of paper spray for two drugs of abuse and metabolites was found to be between 15 and 35% (analyte dependent). For short acquisition times, the extraction efficiency was found to vary between replicates by less than 30%, enabling subsequent analysis by LC/MS. This two‐step process was then applied to fingerprints collected from two patients taking the antipsychotic drug quetiapine, which demonstrates how a negative screening result from paper spray can be resolved using LC/MS. Conclusions: We have shown for the first time the sequential analysis of the same sample using paper spray and LC/MS, as well as the detection of an antipsychotic drug from a fingerprint. We propose that this workflow may also be applied to any type of sample compatible with paper spray, and will be especially convenient where only one sample is available for analysis.