Biomimic Trained Immunity‐MSCs Delivery Microcarriers for Acute Liver Failure Regeneration. Issue 36 (11th April 2022)

• Record Type: Journal Article
• Authors: Wang, Jinglin; Huang, Danqing; Ren, Haozhen; Shang, Luoran
• Is Part Of: Small. Volume 18:Issue 36(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-11
• Subjects: acute liver failure -- biomimicry -- microcarriers -- microfluidics -- trained immunity; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202200858
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Mesenchymal stem cells (MSCs) have a demonstrated value for acute liver failure (ALF) regeneration, while their delivery stratagems with long‐term biological functions, low immune response, and high biocompatibility are still a challenge. Here, a lipopolysaccharide (LPS)‐loaded photoresponsive cryogel porous microcarrier (CPM) for MSCs delivery and colonization is presented to promote defect liver regeneration. The CPMs are fabricated with graphene oxide, poly( N ‐isopropylacrylamide), and gelatin methacrylate (GelMA) via droplet microfluidic technology and a gradient‐cooling procedure. Benefitting from the biocompatible GelMA component and the porous microstructure of the CPMs, MSCs can be nondestructively captured and abundantly delivered. Because the LPS can be released from the CPMs under NIR irradiation, the delivered MSCs are imparted with the feature of "trained immunity." Thus, when the MSCs‐laden CPMs are tailored into the liver matched patches by bioprinting and applied in ALF rats, they display superior anti‐inflammatory and more significant liver regeneration properties than the untrained MSCs. These features make the CPMs an excellent MSCs delivery system for clinical applications in tissue repair. Abstract : Lipopolysaccharide (LPS)‐loaded photoresponsive porous microparticles (CPMs) release LPS under NIR irradiation, where the delivered mesenchymal stem cells (MSCs) are imparted with the feature of "trained immunity." By remodeling the MSCs‐laden CPMs patch through bioprinting in treating acute liver failure rats, this photothermal delivery system displays superior anti‐inflammatory and regeneration ability.