Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses. Issue 10 (24th June 2022)

• Record Type: Journal Article
• Authors: Gong, Jinpeng; Pan, Xiaoyi; Lin, Lingyun; Zhu, Yue; Yao, Jiayun; Wang, Congxu; Yin, Wenlin; Huang, Lei; Liu, Yihan; Chen, Fan; Shen, Jinyu
• Is Part Of: Journal of fish diseases. Volume 45:Issue 10(2022)
• Page Start: 1419
• Page End: 1427
• Publication Date: 2022-06-24
• Subjects: ISKNV -- mandarin fish -- SCRaV -- SCRV -- Siniperca chuatsi -- spinal cord tissue cell line; Fishes -- Diseases -- Periodicals; 639.3
• DOI: 10.1111/jfd.13671
• Languages: English
• ISSNs: 0140-7775

Abstract

Abstract: In this study, we established and characterized a continuous cell line from the spinal cord tissue of mandarin fish, Siniperca chuatsi and assessed its susceptibility to infectious spleen and kidney necrosis virus (ISKNV), Siniperca chuatsi ranavirus (SCRaV) and Siniperca chuatsi rhabdovirus (SCRV). The cell line, named SCC, has been successively cultured up to 40 passages. The optimal growing conditions of SCC cells were in Leibovitz's L‐15 medium supplemented with 20% foetal bovine serum (FBS) at 28°C. Karyotype analysis demonstrated 48 normal diploid chromosomes in the cells. The identity of S. chuatsi origin of SCC cells was confirmed by partial sequencing of the 16S rRNA and cytochrome oxidase I ( COI ) genes. Infection susceptibility assessment showed that ISKNV, SCRIV and SCRV and can be stably produced and transmitted in SCC cells, and the replication efficiency of ISKNV, SCRaV and SCRV ranged from 10 7.4 to 10 9.6 TCID50 /ml. In addition, transmission electron microscopy analysis of ISKNV, SCRAV and SCRV infected SCC cells showed numerous viral particles. In conclusion, the newly established SCC cells provide an important tool for isolation and production of viruses, as well as for molecular and cell biology studies.