Aβ42 oligomers can seed the fibrillization of Aβ40 peptides. Issue 8 (2nd August 2022)

• Record Type: Journal Article
• Authors: Wu, Yi‐Shan; Huang, Shing‐Jong; Wu, Meng‐Hsin; Tu, Ling‐Hsien; Lee, Ming‐Che; Chan, Jerry Chun Chung
• Is Part Of: Journal of the Chinese Chemical Society. Volume 69:Issue 8(2022)
• Page Start: 1318
• Page End: 1325
• Publication Date: 2022-08-02
• Subjects: beta amyloid -- cross seeding -- reverse micelles -- solid‐state NMR; Chemistry -- Periodicals; Electronic journals; 540.5
• DOI: 10.1002/jccs.202200136
• Languages: English
• ISSNs: 0009-4536

Abstract

Abstract: Aggregation of Aβ40 and Aβ42 are considered as pivotal players in the pathogenic mechanism of Alzheimer's disease. In this work, we applied reverse micelles formed by sodium bis(2‐ethylhexyl) sulfosuccinate (AOT) to prepare oligomeric aggregates of Aβ40 or Aβ42 peptides. The resultant globular aggregates were approximately 22 nm in diameter and they were capable to form mature fibrils upon self‐aggregation. Furthermore, we found that the Aβ42 oligomeric aggregates can seed the fibrillization of Aβ40 monomers. Solid‐state NMR results revealed that the Aβ40 fibrils seeded by Aβ40 or Aβ42 oligomers adopt a similar molecular structure for the residues near the C‐terminus. Abstract : The molecular structures of Aβ40 fibrils seeded by Aβ42 oligomers are similar to those seeded by Aβ40 oligomers in the β 2 region.