In vitro and in vivo evaluation of 3D constructs engineered with human iPSC‐derived chondrocytes in gelatin methacryloyl hydrogel. Issue 10 (9th July 2022)

• Record Type: Journal Article
• Authors: Agten, Hannah; Van Hoven, Inge; Viseu, Samuel R.; Van Hoorick, Jasper; Van Vlierberghe, Sandra; Luyten, Frank P.; Bloemen, Veerle
• Is Part Of: Biotechnology and bioengineering. Volume 119:Issue 10(2022)
• Page Start: 2950
• Page End: 2963
• Publication Date: 2022-07-09
• Subjects: cartilage -- GelMA -- iPSC‐derived chondrocytes -- tissue engineering; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.28168
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: Articular cartilage defects have limited healing potential and, when left untreated, can lead to osteoarthritis. Tissue engineering focuses on regenerating the damaged joint surface, preferably in an early stage. Here, we investigate the regenerative potential of three‐dimensional (3D) constructs consisting of human induced pluripotent stem cell (iPSC)‐derived chondrocytes in gelatin methacryloyl (GelMA) hydrogel for stable hyaline cartilage production. iPSC‐derived chondrocytes are encapsulated in GelMA hydrogel at low (1 × 10 7 ml −1 ) and high (2 × 10 7 ml −1 ) density. In a conventional medium, GelMA hydrogel supports the chondrocyte phenotype, as opposed to cells cultured in 3D in absence of hydrogel. Moreover, encapsulated iPSC‐derived chondrocytes preserve their in vivo matrix formation capacity after 21 days in vitro. In differentiation medium, hyaline cartilage‐like tissue forms after 21 days, demonstrated by highly sulfated glycosaminoglycans and collagen type II. Matrix deposition is delayed at low encapsulation density, corroborating with lower transcript levels of COL2A1. An ectopic assay in nude mice demonstrates further maturation of the matrix deposited in vitro. Direct ectopic implantation of iPSC‐derived chondrocyte‐laden GelMA, without in vitro priming, also generates hyaline cartilage‐like tissue, albeit less mature. Since it is unclear what maturity upon implantation is desired for joint surface regeneration, this is an attractive technology to generate immature and more mature hyaline cartilage‐like tissue. Abstract : Based on in vitro and in vivo studies, Agten et al. describe the potential of combining iPSC‐derived chondrocytes with gelatin methacryloyl (GelMA) hydrogel for the robust fabrication of hyaline‐like cartilage building blocks with different degrees of maturity. Additionally, the authors highlight the advantage of using a hydrogel as supporting structure, as iPSC‐derived chondrocytes maintain more phenotypic traits when encapsulated in a GelMA hydrogel and even maintain their in vivo cartilage formation potential after 21 days in conventional medium. Scale bar: 200 µm.