Clinical utilization of chromosomal microarray analysis for the genetic analysis in subgroups of pregnancy loss. (17th November 2022)

• Record Type: Journal Article
• Authors: Gou, Lingshan; Liu, Tianya; Wang, Yi; Wu, Qin; Hu, Shunan; Dong, Bulian; Wang, Chuanxia; Zhang, Yan; Shan, Xinghu; Wang, Xiaona; Suo, Feng; Gu, Maosheng
• Is Part Of: Journal of maternal-fetal & neonatal medicine. Volume 35:Number 22(2022)
• Page Start: 4404
• Page End: 4411
• Publication Date: 2022-11-17
• Subjects: Pregnancy loss -- chromosomal microarray analysis -- copy number variant -- maternal age -- adverse pregnancy history; Obstetrics -- Periodicals; Perinatology -- Periodicals; Infants (Newborn) -- Diseases -- Periodicals; Neonatology -- Periodicals; 618.2
• DOI: 10.1080/14767058.2020.1849126
• Languages: English
• ISSNs: 1476-7058

Abstract

Abstract: Objective: The underlying etiologies of pregnancy loss are heterogeneous and in many cases unexplained. This study was to explore the genetic causes of early and late pregnancy loss using chromosomal microarray analysis (CMA). Methods: A cohort of 222 specimens of conceptions underwent genetic analysis using Affymetrix CytoScan 750 K arrays, which includes both SNP markers and copy number markers. Results: Of the 222-products of conception (POC), the overall detection rate for clinical significantly chromosomal anomalies was 40.54%, including 53 autosomal aneuploidy (23.87%), 16 sex chromosome aneuploidy (7.21%), 5 mutiple aneuploidy (2.25%), 4 triploidy (1.80%), and 12 pathogenic copy number variants (pCNVs) (5.41%). In addition, variants of uncertain significance and loss of heterozygosity were detected in 9 samples and 2 samples, respectively. The detection rates for total chromosomal abnormalities, autosomal aneuploidy, sex chromosome aneuploidy, multiple aneuploidy, and triploidy in specimens of early pregnancy loss was higher than that of late pregnancy loss, while had lower detection rate of pCNVs. Moreover, the detection rate in POC of mothers younger than 35 years was lower than that of advanced maternal age. The detection rate was 40.57% in POC of mothers with adverse pregnancy histories, in which was comparable with that of mothers without adverse pregnancy histories. Conclusions: CMA yielded a superior detection rate in early pregnancy loss than that of late pregnancy loss. Moreover, the incidence of chromosome abnormality in cases with advanced maternal age was higher than that of cases with younger maternal age, while adverse pregnancy history seemed not to be the factors affecting the detection rate for chromosomal abnormality in pregnancy loss.