Mitochondrial DNA sequence of the horseshoe crab Tachypleus gigas. Issue 6 (3rd June 2021)

• Record Type: Journal Article
• Authors: Sarmiento, Maria E.; Chin, Kai Ling; Lau, Nyok Sean; Aziah, Ismail; Norazmi, Mohd Nor; Acosta, Armando; Ismail, Noraznawati; Yaacob, Nik Soriani
• Is Part Of: Mitochondrial DNA. Volume 6:Issue 6(2021)
• Page Start: 1710
• Page End: 1714
• Publication Date: 2021-06-03
• Subjects: Tachypleus gigas -- mitochondrial genome -- phylogenetic analysis -- whole genome sequencing -- Terengganu -- Malaysia; Mitochondrial DNA -- Periodicals; 572.869
• DOI: 10.1080/23802359.2021.1930213
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: This paper reports on the complete mitochondrial (mt) genome of a horseshoe crab, Tachypleus gigas ( T. gigas ), in Kuala Kemaman, Terengganu, Malaysia. Whole-genome sequencing of hemocyte DNA was performed with Illumina HiSeq system and the generated reads were de novo assembled with ABySS 2.1.5 and reassembled using mitoZ against Carcinoscorpius rotundicauda and Limulus polyphemus, resulting in a contig of 15 Kb. Phylogenetic analysis of the assembled mt genome suggests that the Tachypleus gigas is closely related to Tachypleus tridentatus than to Carcinoscorpius rotundicauda.