Antioxidant Activity and Polyphenols Content of Artemisia Herba-Alba Extract and their Cytotoxicity against Human Lung Cancer Cells NCI-N417. Issue 4 (2nd October 2022)

• Record Type: Journal Article
• Authors: Amkiss, Sanae; Bakha, Mohamed; Belmehdi, Omar; Carmona-Espinazo, Fernando; López-Sáez, Juan-Bosco; Idaomar, Mohamed
• Is Part Of: Journal of herbs, spices & medicinal plants. Volume 28:Issue 4(2022)
• Page Start: 337
• Page End: 350
• Publication Date: 2022-10-02
• Subjects: Antiproliferative -- flow cytometry; Medicinal plants -- Periodicals; Herbs -- Periodicals; Spices -- Periodicals; 581.63
• DOI: 10.1080/10496475.2022.2064954
• Languages: English
• ISSNs: 1049-6475

Abstract

ABSTRACT: Phytochemicals in the ethanol extract of Artemisia herba-alba were examined and evaluated for the antioxidant and cytotoxicity potential. The extraction was performed using an accelerated solvent extraction (ASE300), analyzed by GC-MS and the total phenolics was estimated by Folin–Ciocalteau method. The antioxidant activity of the extract was evaluated using three methods and its cytotoxicity was evaluated in-vitro on NCI-N417 tumor cell line using MTT test and flow cytometry. Twenty-one compounds were identified in the A. herba-alba extract, which was rich in phenolic compounds (248.56 ± 11.05 μg GAE mg −1 extract) and had antioxidant activity characterized by a high DPPH-radical-scavenging activity (IC50 = 69.01 ± 0.03 μg mL −1 ), inhibitory effect of β-carotene oxidation (95.75%±0.013), and reducing power (DO700nm = 0.751 ± 0.001). Ethanol extract from A. herba-alba demonstrated cytotoxic and antiproliferative activity.