Quantity and Quality Test of DNA Marigold Plants (Tagetes erecta L.) for the Suistainability of Plant Breeding. Issue 1 (1st June 2022)

• Record Type: Journal Article
• Authors: Pangaribuan, O; Hanafiah, D S; Setiado, H; Simamora, J R M; Sari, N W
• Is Part Of: IOP conference series. Volume 977:Issue 1(2022)
• Publication Date: 2022-06-01
• Subjects: Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/977/1/012047
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: Marigold is one of the potential commercial flowers and demand continues toincrease. The high demand for marigold plants among the community so that the need for mutation plant breeding for marigold plants. It is hoped that giving mutations, it can increase genetic diversity in marigold plants which will be used as material for plant breeding activities to form new superior marigold varieties. This study aimed to examine the quantity and quality of mutant DNA marigold plants for the suistainabiliy of plant breeding. This research was conducted in the molecular genetics laboratory, Faculty of Agriculture, Universitas Sumatera Utara. The DNA of 5 mutant samples of marigold plants was isolated using the CTAB extraction method. The results showed that the lines that had bright and thick band patterns were found in samples P0, P2, P3 and P4 while thin and less bright bands were found insample P1. The purity of the resulting DNA ranged from 1.84 to 2.00 and the concentration of the resulting DNA ranged from 30.2 to 53.7 g/ml.