Contribution of prgH gene for Salmonella Pullorum to virulence and the expression of NLRP3, Caspase-1 and IL-1β in chickens. (October 2022)

• Record Type: Journal Article
• Authors: Yin, Junlei; Cheng, Zhao; Xu, Zhenyu; Zhi, Lijuan; Zhang, Yige; Yuan, Xinzhong; Pan, Pengtao; Sun, Weiwei; Yu, Tao; Liu, Tiantian
• Is Part Of: Microbial pathogenesis. Volume 171(2022)
• Publication Date: 2022-10
• Subjects: Sallmonella pullorum -- prgH -- Virulence -- NLRP3 -- Caspase-1 -- IL-1β; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105744
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Type III secretion system 1 (T3SS1) encoded by Salmonella pathogenicity island 1 (SPI1) is associated with invasion of host cells by Salmonella, PrgH encoded by prgH gene is an important component of T3SS1. This study aimed to explore the contribution of prgH gene for Salmonella Pullorum to virulence and the expression of NLRP3, Caspase-1 and IL-1β in chickens. A prgH gene deletion mutant (C79-13Δ prgH ) was firstly generated, and the result of LD50 showed that deletion of prgH significantly decreased the virulence of Salmonella Pullorum in one-day-old HY-line white chickens, and the colonization also decreased in chickens after loss of prgH . Next, the expressions of NLRP3, Caspase-1, and IL-1β were detected in acute infection model of chickens by qRT-PCR and/or ELISA, respectively, and the results showed that the mutant strain C79-13Δ prgH reduced the expression levels of NLRP3, Caspase-1, and IL-1β in chickens compared to the group infected with the wild type strain C79-13. Taken together, all of these findings indicated that prgH promotes the virulence and the expression of NLRP3, Caspase-1, and IL-1β for Salmonella Pullorum in chickens. Highlights: A prgH deletion mutant of Salmonella Pullorum was successfully constructed. The virulence of the prgH mutant was significantly decreased. The prgH gene promotes colonization of Salmonella Pullorun and expression of NLRP3, Caspase-1 and IL-1β in chickens. Salmonella Pullorum prgH gene takes part in the regulation of inflammatory response in chickens.