Formation mechanisms and assembly patterns of anammox biofilm induced by carrier type: Novel insights based on low-strength wastewater treatment. (October 2022)

• Record Type: Journal Article
• Authors: Wang, XiaoTong; Yang, Hong; Liu, XuYan; Wang, JiaWei
• Is Part Of: Bioresource technology. Volume 362(2022)
• Publication Date: 2022-10
• Subjects: Anammox -- Polyurethane sponge carrier -- Aggregation mechanism -- Extracellular polymeric substances -- Metagenomic analysis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127863
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: The carrier anisotropic microenvironment is key to inducing the biofilm center model. Biofilms without carrier induction have a higher inorganic mass ratio and strength. Large-pore polyurethane carrier biofilms have high independent stability. Candidatus Brocadia sinica is more competitive under low matrix short HRT conditions. Rm has more abundant heterotrophic bacteria and supports strong metabolic function. Abstract: The morphological structure, properties, microbial community and function of anammox biofilms induced by large-pore carriers (Bls), small-pore carriers, filament carriers and non-carriers (Bn) in low-strength wastewater were comprehensively studied. The carriers promoted biomass accumulation and agglomeration, with Bls demonstrating the highest biomass proportion of 0.76, the highest specific anammox activity (0.41 kgN/(kgVSS·d) −1 ) and the largest aggregates. Hydraulic shearing stimulated Bn to secrete most extracellular polymeric substances and capture more inorganic ions for enhanced strength. Metagenomic sequencing showed that the four biofilms shared a common core flora, but differed in cross-metabolism. The proportion of the functional bacterium Candidatus Brocadia was highest in Bls, while the increase in heterotrophic bacteria in Bn supported stronger metabolic capacity. Finally, the proposed anisotropic or isotropic carrier structure was identified as the key to generating "uniform development" and "central development" models. This study is helpful for understanding the anammox aggregation mechanism and carrier optimization.