Efficacy of IL10/STAT3 directed small molecule immunotherapy in augmenting the potential of rBCG30 vaccine against murine pulmonary tuberculosis. (October 2022)

• Record Type: Journal Article
• Authors: Ahmad, Faraz; Umar, Mohd. Saad; Zubair, Swaleha; Khan, Nazoora; Gupta, Pushpa; Gupta, Umesh Datta; Owais, Mohammad
• Is Part Of: Molecular immunology. Volume 150(2022)
• Page Start: 14
• Publication Date: 2022-10
• Subjects: Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2022.05.053
• Languages: English
• ISSNs: 0161-5890

Abstract

Abstract : Several recent studies have contributed to our understanding of the need for host directed immunotherapeutic strategies against Mycobacterium tuberculosis ( Mtb ), either concomitantly with classical chemotherapy or also as standalone approach. Nevertheless, most of these studies were focused on enhancing chemotherapeutic potential of standard anti-tuberculosis chemotherapy and only few were aimed towards improving prophylactic immunity offered either by BCG or its potential replacements. In the present study we tried to address the potential gap with the aim to improve anti-TB immunity offered by rBCG30 vaccine. We attempted to bolster rBCG30 invoked anti-TB immunity in mice through modulation of the IL-10/STAT3 interaction mediated anti inflammatory program. An *immunomodulator of IL-10/STAT3 signaling axis was administered in immunized mice to reinforce the anti pathogen capabilities of mononuclear phagocyte system and thereby, to improve T cell mediated secondary immune responses. We envisage that modulation of this crucial praxis may result in reduced expansion of pathogen permissive AAMs/AADCs (Alternatively Activated Monocytes/Macrophages/DCs) and augmented abundance of pro-inflammatory CAMs/CADCs (Classically Activated Monocytes/Macrophages/DCs), the cells that resist establishment of successful Mtb infection. The study was also aimed towards generating optimum activated APCs early either after vaccination or infection, which can process and present mycobacterial antigens more efficiently to T cells.