Evaluation of Various Silicon- and Boron-Containing Compounds for the Detection of Phosphorylation in Peptides via Gas-Phase Ion-Molecule Reactions. Issue 4 (August 2014)

• Record Type: Journal Article
• Authors: Piatkivskyi, Andrii; Pyatkivskyy, Yuriy; Ryzhov, Victor
• Is Part Of: European journal of mass spectrometry. Volume 20:Issue 4(2014)
• Page Start: 337
• Page End: 344
• Publication Date: 2014-08
• Subjects: quadrupole ion trap -- ion–molecule reactions -- phosphopeptides -- trimethyl borate -- triethyl borate -- diethylmethoxyborane -- diisopropoxymethylborane -- chlorotrimethylsilane -- N, O-bis(trimethylsilyl)acetamide; Mass spectrometry -- Periodicals; Mass Spectrometry; Mass spectrometry; Periodicals; Periodicals; 543.6505
• DOI: 10.1255/ejms.1286
• Languages: English
• ISSNs: 1469-0667

Abstract

Gas-phase ion–molecule reactions (IMR) of various boron- and silicon-containing neutrals were investigated as a potential route for detecting phosphorylation within peptides in the negative ion mode. Trimethyl borate (TMB), triethyl borate (TEB) and N, O -Bis(trimethylsilyl)acetamide (TMSA), unlike diethylmethoxyborane (DEMB), diisopropoxymethylborane (DiPMB) and chlorotrimethylsilane (TMSCl), reacted differently if a phosphate moiety was present and thus are suitable to detect phosphorylation. During multistage collision-induced dissociation experiments of the reaction products of IMR with TMB and TEB, the [LSsF − 4H + B] − ion formed a modified y2 * fragment allowing the phosphorylation site to be assigned, unlike reaction products of DEMB and DiPMB which lost both the phosphoric acid and the boron-containing moiety.