Genetic dissection of natural variation in oilseed traits of camelina by whole‐genome resequencing and QTL mapping. Issue 2 (9th June 2021)

• Record Type: Journal Article
• Authors: Li, Huang; Hu, Xiao; Lovell, John T.; Grabowski, Paul P.; Mamidi, Sujan; Chen, Cindy; Amirebrahimi, Mojgan; Kahanda, Indika; Mumey, Brendan; Barry, Kerrie; Kudrna, David; Schmutz, Jeremy; Lachowiec, Jennifer; Lu, Chaofu
• Is Part Of: œplant genome. Volume 14:Issue 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-06-09
• Subjects: Plant genomes -- Periodicals; Plant genome mapping -- Periodicals; 572.862
• DOI: 10.1002/tpg2.20110
• Languages: English
• ISSNs: 1940-3372

Abstract

Abstract: Camelina [ Camelina sativa (L.) Crantz] is an oilseed crop in the Brassicaceae family that is currently being developed as a source of bioenergy and healthy fatty acids. To facilitate modern breeding efforts through marker‐assisted selection and biotechnology, we evaluated genetic variation among a worldwide collection of 222 camelina accessions. We performed whole‐genome resequencing to obtain single nucleotide polymorphism (SNP) markers and to analyze genomic diversity. We also conducted phenotypic field evaluations in two consecutive seasons for variations in key agronomic traits related to oilseed production such as seed size, oil content (OC), fatty acid composition, and flowering time. We determined the population structure of the camelina accessions using 161, 301 SNPs. Further, we identified quantitative trait loci (QTL) and candidate genes controlling the above field‐evaluated traits by genome‐wide association studies (GWAS) complemented with linkage mapping using a recombinant inbred line (RIL) population. Characterization of the natural variation at the genome and phenotypic levels provides valuable resources to camelina genetic studies and crop improvement. The QTL and candidate genes should assist in breeding of advanced camelina varieties that can be integrated into the cropping systems for the production of high yield of oils of desired fatty acid composition. Core Ideas: Genome resequencing and population structure analyses are used to assess genetic diversity. QTL mapping is conducted to identify regions associated with seed traits in camelina. Genomic resources and candidate genes are provided to improve camelina seed traits.