In situ microcosms deployed at the coast of British Columbia (Canada) to study dilbit weathering and associated microbial communities under marine conditions. Issue 7 (14th June 2021)

• Record Type: Journal Article
• Authors: Schreiber, Lars; Fortin, Nathalie; Tremblay, Julien; Wasserscheid, Jessica; Sanschagrin, Sylvie; Mason, Jennifer; Wright, Cynthia A; Spear, David; Johannessen, Sophia C; Robinson, Brian; King, Thomas; Lee, Kenneth; Greer, Charles W
• Is Part Of: FEMS microbiology ecology. Volume 97:Issue 7(2021)
• Publication Date: 2021-06-14
• Subjects: dilbit -- oil biodegradation -- Douglas Channel -- Hecate Strait -- in situ microcosms; Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1093/femsec/fiab082
• Languages: English
• ISSNs: 0168-6496

Abstract

ABSTRACT: Douglas Channel and the adjacent Hecate Strait (British Columbia, Canada) are part of a proposed route to ship diluted bitumen (dilbit). This study presents how two types of dilbit naturally degrade in this environment by using an in situ microcosm design based on dilbit-coated beads. We show that dilbit-associated n-alkanes were microbially biodegraded with estimated half-lives of 57–69 days. n-Alkanes appeared to be primarily degraded using the aerobic alk B, lad A and CYP153 pathways. The loss of dilbit polycyclic aromatic hydrocarbons (PAHs) was slower than of n-alkanes, with half-lives of 89–439 days. A biodegradation of PAHs could not be conclusively determined, although a significant enrichment of the phnAc gene (a marker for aerobic PAH biodegradation) was observed. PAH degradation appeared to be slower in Hecate Strait than in Douglas Channel. Microcosm-associated microbial communities were shaped by the presence of dilbit, deployment location and incubation time but not by dilbit type. Metagenome-assembled genomes of putative dilbit-degraders were obtained and could be divided into populations of early, late and continuous degraders. The majority of the identified MAGs could be assigned to the orders Flavobacteriales, Methylococcales, Pseudomonadales and Rhodobacterales . A high proportion of the MAGs represent currently unknown lineages or lineages with currently no cultured representative. Abstract : This study investigated the biodegradation of diluted bitumen and the associated microbial communities by using a novel in situ microcosm design deployed at the coast of British Columbia (Canada).