Evaluating the effectiveness of anti-tuberculosis treatment by detecting Mycobacterium tuberculosis 85B messenger RNA expression in sputum. Issue 10 (October 2020)

• Record Type: Journal Article
• Authors: Atahan, Ersan; Saribas, Suat; Demirci, Mehmet; Babalık, Aylin; Akkus, Seher; Balıkcı, Ahmet; Satana, Dilek; Ziver, Tevhide; Dinc, Harika Oyku; Keskin, Melike; Ozbey, Dogukan; Kocak, Banu Tufan; Gareayaghi, Nesrin; Kirmusaoglu, Sahra; Tokman, Hrisi Bahar; Kocazeybek, Bekir
• Is Part Of: Journal of infection and public health. Volume 13:Issue 10(2020)
• Page Start: 1490
• Page End: 1494
• Publication Date: 2020-10
• Subjects: 85B mRNA -- Mycobacterium tuberculosis -- Anti-tuberculosis treatment; Communicable diseases -- Periodicals; Public health -- Periodicals; Epidemiology -- Periodicals; Nosocomial infections -- Prevention -- Periodicals; Medical microbiology -- Periodicals; 614.4
• DOI: 10.1016/j.jiph.2020.05.016
• Languages: English
• ISSNs: 1876-0341

Abstract

Abstract: Background: The antigen 85 complex (85B) is secreted in large quantities from growing mycobacteria and the presence of bacterial mRNA is an indicator of cell viability. The quantitative detection of 85B mRNA expression levels can be used to assess the success of anti-tuberculosis treatment outcomes to detect viable mycobacteria cells. Therefore, we evaluated the levels of 85B mRNA of Mycobacterium tuberculosis strains in patients with pulmonary tuberculosis. Methods: Thirty patients with primary tuberculosis were included in this study. The sputum specimens of patients were collected on days 0, 15, and 30 days and were cultured and evaluated by 85B mRNA-based RT-qPCR. Results: Overall, 23 of the studied tuberculosis strains were susceptible to the primary anti-tuberculosis antibiotics used in this study, 7 were resistant. By the 30th day of treatment, 85B mRNA was detected in only one of the susceptible strains, but in all 7 of the resistant strains, though the relative gene expression varied between the strains. This difference between the susceptible and resistant strains at day 30 was statistically significant (p < 0.05). Conclusion: 85B mRNA expression levels could be used to follow up on primary tuberculosis cases. 85B mRNA seems to be a good diagnostic marker for monitoring anti-tuberculosis treatment outcomes.