Analysis of the differential metabolic pathway of cultured Chlorococcum humicola with hydroquinone toxic sludge extract. (10th October 2022)

• Record Type: Journal Article
• Authors: Yang, Yingying; Zhang, Xinyu; Hu, Xueyang; Zhao, Jiamin; Chen, Xiurong; Wei, Xiao; Yu, Xiao
• Is Part Of: Journal of cleaner production. Volume 370(2022)
• Publication Date: 2022-10-10
• Subjects: Sludge toxicity -- Chlorococcum humicola -- protein Interaction -- Hydroquinone -- Proteomics analysis; Factory and trade waste -- Management -- Periodicals; Manufactures -- Environmental aspects -- Periodicals; Déchets industriels -- Gestion -- Périodiques; Usines -- Aspect de l'environnement -- Périodiques; 628.5
• DOI: 10.1016/j.jclepro.2022.133486
• Languages: English
• ISSNs: 0959-6526

Abstract

Abstract: Microbe inhibition produces intermediate metabolites and toxic substances that accumulate significantly in residual sludge toxicity after continuous treatment of coal gasification wastewater with activated sludge. To address this issue, Chlorococcum humicola was cultured with sludge extract. Proteomics analysis was used to investigate the mechanism of stress response in microalgae to sludge toxicity. Upon completion of growth, microalgae treated with sludge extract produced 0.725 ± 0.036 g/L of biomass. The total organic carbon concentration and toxicity removal rates on microalgae were 75.1 ± 3.8% and 47.4 ± 2.1%, respectively. The proteomics analysis revealed that under stress conditions, microalgae altered cellular components such as the nucleus and plasma membranes, affecting the photosynthetic and respiratory processes. The logarithmic phase was characterized by a down-regulation of oxidative phosphorylation and an increase in energy synthesis regulated by the isopropyl phosphate isomerase. The results demonstrate a proteomic basis for the reduction and utilization of waste sludge, as well as provide a molecular mechanism reference for optimizing the model of cultivating microalgae using sludge. Graphical abstract: Image 1 Highlights: Sludge extract from hydroquinone treatment was utilized to cultivate microalgae. The optimal cultivation ratio would increase biomass production and TOC disposal. Oxidative phosphorylation was controlled downwards in the logarithmic phase. Energy synthesis and biosynthesis have been adjusted under toxic stress. Abstract : Synopsis Sludge extract was used to reduce sludge toxicity and increase biomass accumulation in Chlorococcum humicola . Stress altered the nucleus and plasma membranes of microalgae.