Brevinin-2PN, an antimicrobial peptide identified from dark-spotted frog (Pelophylax nigromaculatus), exhibits wound-healing activity. (December 2022)

• Record Type: Journal Article
• Authors: Fan, Xiao-Li; Yu, Shui-Sheng; Zhao, Jia-Le; Li, Yue; Zhan, Du-Juan; Xu, Feng; Lin, Zhi-Hua; Chen, Jie
• Is Part Of: Developmental and comparative immunology. Volume 137(2022)
• Publication Date: 2022-12
• Subjects: Antimicrobial peptides -- Dark-spotted frog -- Brevinin-2 -- Antibacterial activity -- Wound-healing activity; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2022.104519
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Brevinins exhibit a wide range of structural features and strong biological activities. Brevinin-2, derived from several amphibians, has shown antimicrobial activities. However, little is known about the wound-healing activity of brevinin-2. In this study, brevinin-2 cDNA was identified from the skin transcriptome of the dark-spotted frog ( Pelophylax nigromaculatus ) and it comprises a signal peptide, a propeptide, and a mature peptide. Sequence alignment with brevinin-2 derived from other amphibians showed variability of the mature peptide, and the presence of a C-terminal cyclic heptapeptide domain (Cys-Lys-Xaa4-Cys) in the mature peptide. Dark-spotted frog brevinin-2 belonged to the brevinin-2 cluster and was closely related to brevinin-2HB1 from Pelophylax hubeiensis . Synthetic dark-spotted frog brevinin-2 mature peptide (brevinin-2PN) exhibited antibacterial activity against several pathogens by destroying cell membrane integrity and hydrolysis of genomic DNA. Brevinin-2PN exhibited significant wound-healing activity by accelerating the healing of human skin fibroblast cell scratches, influencing cell migration, and stimulating gene expression of growth factors. Highlights: We cloned a brevinin-2 gene from the dark-spotted frog Pelophylax nigromaculatus. Brevinin-2PN exhibited antibacterial activity against several pathogens. Significant wound-healing activity in HSF cells by brevinin-2PN was observed.