Anchoring the ethylene-forming enzyme to photosystem-related proteins to improve ethylene production in engineered Synechocystis PCC 6803. (September 2022)

• Record Type: Journal Article
• Authors: Gao, Xiang; Liu, Xufeng; Jing, Xin; Lindblad, Peter
• Is Part Of: Bioresource technology reports. Volume 19(2022)
• Publication Date: 2022-09
• Subjects: Ethylene -- Metabolic engineering -- Protein anchoring -- Photosystem -- cyanobacteria; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2022.101178
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: Ethylene production in engineered cyanobacteria offers a sustainable alternative to the traditional production from fossil resources. Protein fusions and anchoring strategies are promising routes to augment the stability of recombinant proteins. Here we attempted to anchor the ethylene-forming enzyme (Efe) to several photosystem-related proteins (including D1, PetF, and PsaE) in order to improve the ethylene producing capability in engineered cells of Synechocystis PCC 6803. Generated strains harboring fused Efe with D1 or PetF showed respectively 1.6- and 1.5-fold increase in ethylene production compared to a control strain harboring only Efe. Additionally, strains co-expressing a non-fused Efe with PsaE or PetF showed respectively 3.5- and 3.1-fold increase in ethylene production compared to the control strain. This work demonstrates the feasibility to incorporate (anchor or co-express) target enzyme(s) with specific photosystem components for facilitating highly efficient production of bulk chemicals in engineered cyanobacteria. Graphical abstract: Unlabelled Image Highlights: Ethylene-forming enzyme was anchored/co-expressed with photosystem-related proteins. Engineered strains harboring anchoring constructs showed high ethylene production. Developed strains harboring co-expression constructs produced even more ethylene.