A stressing method for producing high-density Trichoderma spores in a dual-layer by utilizing a starch-based medium in a reconditioning approach. (September 2022)

• Record Type: Journal Article
• Authors: Abuhena, Md.; Kabir, Md. Golam; Azim, Md. Faisal; Al-Rashid, Jubair; Rasul, Noorain Munim; Huq, Md. Amdadul
• Is Part Of: Bioresource technology reports. Volume 19(2022)
• Publication Date: 2022-09
• Subjects: Trichoderma harzianum -- Sporulation -- Fungal media -- Parameters optimization -- Industrial production -- Clogging; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2022.101165
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: A stressing method was adopted to produce Trichoderma harzianum TI21 spores in a dual-layer with an outer-layer spore free of media-residue by reconditioning the starch-based rice agar medium. The essential parameters were explored and optimized using statistical methods. The single factorial test's findings demonstrated that lower-pH and starch-based media encouraged sporulation while suppressing the growth of contaminants. The Box-Behnken design identified the ideal levels as pH 4, 30 °C, and 75 %. In the reconditioning approach, the first harvest produced 4.91 (3.64 × 10 11 CFU/g) of spores (g/L) after 5 days of cultivation, while the second and third harvests produced 4.19 and 3.48 (respectively) after 4 days of cultivation, leaving 755 (g/L) (2.25 × 10 10 CFU/g) media-mixed spores. As compared to media-mixed spores, media residue-free spores had significantly longer viability (18 months) with no clogging hazards. According to the findings, the method can be used industrially to produce stable, high-density spores for widespread agricultural applications. Graphical abstract: Sporulation of Trichoderma harzianum TI21 in a reconditioning approach on starch-based media in the stressing method. Unlabelled Image Highlights: Production technology reduced the cultivation time. Rice agar and low pH suppressed the growth of contaminants. Box-Behnken design is effective for predicting the spore yield. Reconditioning approach maximized the yield of media-free high-density spores. Media-free spores showed longer viability with no clogging effects.