Enhancing hydrogen production from winery wastewater through fermentative microbial culture selection. (September 2022)

• Record Type: Journal Article
• Authors: Policastro, Grazia; Carraturo, Federica; Compagnone, Mariacristina; Guida, Marco; Fabbricino, Massimiliano
• Is Part Of: Bioresource technology reports. Volume 19(2022)
• Publication Date: 2022-09
• Subjects: Biorefinery -- Co-fermentation -- Dark fermentation -- Open mixed culture -- Photo fermentation; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2022.101196
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: The present paper aims at enhancing the fermentative/co-fermentative hydrogen production from winery wastewater. Winery wastewater is a very abundant waste stream, with a high hydrogen production potential. On the other hand, it might exert toxic effects towards many hydrogen-producing bacteria. To optimize the process, a mixed microbial consortium was subject to microbial analysis to identify hydrogen-producing strains. These latter were isolated to obtain several pure cultures, either dark-fermentative, or photo-fermentative. Comparative fermentation tests were performed, using the isolated pure cultures, the initial mixed culture and a co-culture consisting of the two most performant dark fermentative and photo fermentative strains (i.e. Klebsiella pneumoniae strain MF101 and Rhodopseudomonas sp. strain BR0Y6). Results show that the initial mixed culture led to the highest production of 290 NmLH2 L −1 . Further analyses on metabolic intermediates demonstrate the importance of synergies established in open fermentations to enhance the conversion of complex organic substrates to hydrogen. Graphical abstract: Unlabelled Image Highlights: The optimization of hydrogen production from winery wastewater is proposed. The selection of both dark and photo fermentative bacteria has been performed. Pure cultures, co-cultures and open mixed cultures have been compared. The open mixed culture fermentation enhances hydrogen production. Synergistic effects among microorganisms enhance the conversion of waste.