Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta). Issue 5 (15th July 2022)

• Record Type: Journal Article
• Authors: Smedley, Jeremy V.; Bochart, Rachele M.; Fischer, Miranda; Funderburgh, Heidi; Kelly, Vanessa; Crank, Hugh; Armantrout, Kim; Shiel, Oriene; Robertson‐LeVay, Mitchell; Sternberger, Nikki; Schmaling, Brian; Roberts, Sheila; Sekiguchi, Vicki; Reusz, Michael; Schwartz, Tiah; Meyer, Kimberly A.; Webb, Gabriela; Gilbride, Roxanne M.; Dambrauskas, Nicholas; Andrade, Daniela; Wood, Matthew; Labriola, Caralyn; Axthelm, Michael; Derby, Nina; Varco‐Merth, Ben; Fukazawa, Yoshinori; Hansen, Scott; Sacha, Jonah B.; Sodora, Donald L.; Sather, D. Noah
• Is Part Of: Journal of medical primatology. Volume 51:Issue 5(2022)
• Page Start: 270
• Page End: 277
• Publication Date: 2022-07-15
• Subjects: draining -- fluorescence -- ICG -- imaging -- indocyanine green -- lymph node -- macaque -- mesenteric -- MLN -- targeted sampling; Primates -- Periodicals; Primates -- Diseases -- Periodicals; Medicine, Experimental -- Periodicals; 616.02738
• DOI: 10.1111/jmp.12605
• Languages: English
• ISSNs: 0047-2565

Abstract

Abstract: Background: Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging. Methods: Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging. Results: After optimization to maximize LN identification, intradermal ICG was successful in identifying 50–100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs. Conclusion: These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.